Chemical Synthesis Protocols

Preparation of TLC Stains

Molecular Biology Protocols

Preparation of Competent Cells

Biochemical Protocols

Affinity Purification of Antibodies
Silver Staining of SDS-PAGE Gels

Cell Culture Protocols

Immunofluorescent Staining of Adherent Cells
Luciferase-based Hedgehog Signaling Assays
Luminescent C3HT10(1/2) Differentiation Assay
Microscope Mercury Lamp Alignment

Zebrafish Protocols

Chen Lab Fish Facility Standard Operating Procedures
Bleaching Zebrafish Embryos
Genomic DNA Isolation for PCR Analysis
Manual and Enzymatic Dechorionation of Zebrafish Embryos
Zebrafish Embryo Dissociation
cDNA Preparation from Zebrafish Embryos
cDNA Synthesis and Amplification for Microarrays
Whole-Mount in situ Hybridizations
Whole-Mount Antibody Staining